|Professor of Ophthalmology & Visual Science
Adjunct Professor of Neurobiology and Anatomy
Adjunct Professor of Bioengineering
The Krizaj Lab
Moran Eye Center lab link
Calcium regulation and polymodal signaling in the retina
David Krizaj's laboratory is interested in the relationship between intracellular signaling pathways and neurotransmission in the retina. The lab is interested in how non-conventional signaling pathways such as intracellular calcium stores, calcium transporters and store-operated calcium channels collaborate with voltage-operated signals to modulate graded exocytosis. For example, recent experiments elucidated the roles of ryanodine receptors, mitochondria and TRPC channels in spatiotemporal calcium signaling in photoreceptors, glial cells and retinal ganglion neurons. Other projects focus on how TRP channels integrate temperature, mechanical stress and lipid signals, and how translation of these sensory inputs impacts on parallel light-evoked inputs and survival in ganglion cells. A third project is focused on using in vivo multiphoton imaging to define the physiology of microglia within the retina.
Jo, A.O., Lakk, M., Frye, A.M., Phuong, T.T.T., Berkowitz, B., Yarishkin, O., and Krizaj, D. (2016) Differential osmoregulation and calcium signaling in two ciliary body cell types is subserved by TRPV4 channels. Proceedings of the National Academy of Sciences U.S.A., Mar 22.
Molnar, T.*, Yarishkin, O.*, Iuso, A., Barabas, P., Jones, B., Marc, R.E., Phuong, T.T., and Krizaj, D. (2016) Store-operated calcium entry in Müller glia is controlled by synergistic activation of TRPC and Orai channels. Journal of Neuroscience, 36(11):3184-3198.
Jo, A.O.*, Ryskamp, D.A.*, Phuong, T.T.T., Verkman, A., Yarishkin, O., MacAulay, N., and Krizaj, D. (2015) Synergistic signaling of TRPV4 and AQP4 channels is required for cell volume and calcium homeostasis regulation in retinal Müller glia. Journal of Neuroscience, 35(39):13525-13537.
Ryskamp, D.A., Frye, A., Macaulay, N., Vazquez-Chona, F., Thoreson, W.B., and Krizaj, D. (2014) Swelling and eicosanoid metabolites differentially gate TRPV4 channels in retinal neurons and glia. Journal of Neuroscience, 34(47):15689-15700. PMID: 25411497
Chen, M., Krizaj, D., and Thoreson, W.B. (2014) Intracellular stores drive sustained non-ribbon vesicle release from rod photoreceptors. Frontiers in Cellular Neuroscience, 8:20 PMID: 24550779
Barabas, P., Liu, A., Xing, W., Watt, C., Cheng, J.K., Tong, Z., Jones, B.W., Bernstein, P., and Krizaj, D. (2013) The Stargardt-3 allele of ELOVL4, but not photoreceptor cell-specific elimination of Elovl4, triggers a juvenile progressive loss of visual function in the mouse. Proceedings of the National Academy of Sciences U.S.A., 110(13):5181-5186 PMID: 23479632
Molnar, T., Barabas, P., Birnbaumer, L., Punzo, C., Kefalov, V., and Krizaj, D. (2012) Store-operated channels regulate intracellular calcium in mammalian rods. Journal of Physiology, 590:3465-3481 PMID: 22674725